The high-affinity poplar ammonium importer PttAMT1.2 and its role in ectomycorrhizal symbiosis.
Identifieur interne : 003F39 ( Main/Exploration ); précédent : 003F38; suivant : 003F40The high-affinity poplar ammonium importer PttAMT1.2 and its role in ectomycorrhizal symbiosis.
Auteurs : Anita Selle [Allemagne] ; Martin Willmann ; Nina Grunze ; Arthur Gessler ; Michael Weiss ; Uwe NehlsSource :
- The New phytologist [ 0028-646X ] ; 2005.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Composés d'ammonium quaternaire (métabolisme), Croisements génétiques (MeSH), Mycorhizes (physiologie), Populus (croissance et développement), Populus (microbiologie), Protéines végétales (génétique), RT-PCR (MeSH), Réaction de polymérisation en chaîne (MeSH), Symbiose (MeSH), Séquence nucléotidique (MeSH), Transport biologique (MeSH), Transporteurs de cations (génétique).
- MESH :
- croissance et développement : Populus.
- génétique : Protéines végétales, Transporteurs de cations.
- microbiologie : Populus.
- métabolisme : Composés d'ammonium quaternaire.
- physiologie : Mycorhizes.
- Amorces ADN, Croisements génétiques, RT-PCR, Réaction de polymérisation en chaîne, Symbiose, Séquence nucléotidique, Transport biologique.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Biological Transport (MeSH), Cation Transport Proteins (genetics), Crosses, Genetic (MeSH), DNA Primers (MeSH), Mycorrhizae (physiology), Plant Proteins (genetics), Polymerase Chain Reaction (MeSH), Populus (growth & development), Populus (microbiology), Quaternary Ammonium Compounds (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , genetics : Cation Transport Proteins, Plant Proteins.
- growth & development : Populus.
- chemical , metabolism : Quaternary Ammonium Compounds.
- microbiology : Populus.
- physiology : Mycorrhizae.
- Base Sequence, Biological Transport, Crosses, Genetic, DNA Primers, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Symbiosis.
Abstract
One way to elucidate whether ammonium could act as a nitrogen (N) source delivered by the fungus in ectomycorrhizal symbiosis is to investigate plant ammonium importers. Expression analysis of a high-affinity ammonium importer from Populus tremulax tremuloides (PttAMT1.2) and of known members of the AMT1 gene family from Populus trichocarpa was performed. In addition, PttAMT1.2 function was studied in detail by heterologous expression in yeast. PttAMT1.2 expression proved to be root-specific, affected by N nutrition, and strongly increased in a N-independent manner upon ectomycorrhiza formation. The corresponding protein had a K(M) value for ammonium of c. 52 microm. From the seven members of the AMT1 gene family, one gene was exclusively expressed in roots while four genes were detectable in all poplar organs but with varying degrees of expression. Ectomycorrhiza formation resulted in a strong upregulation of three of these genes. Our results indicate an increased ammonium uptake capacity of mycorrhized poplar roots and suggest, together with the expression of putative ammonium exporter genes in the ectomycorrhizal fungus Amanita muscaria, that ammonium could be a major N source delivered from the fungus towards the plant in symbiosis.
DOI: 10.1111/j.1469-8137.2005.01535.x
PubMed: 16313651
Affiliations:
Links toward previous steps (curation, corpus...)
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type="city">Tübingen</settlement></placeName></affiliation></author><author><name sortKey="Willmann, Martin" sort="Willmann, Martin" uniqKey="Willmann M" first="Martin" last="Willmann">Martin Willmann</name></author><author><name sortKey="Grunze, Nina" sort="Grunze, Nina" uniqKey="Grunze N" first="Nina" last="Grunze">Nina Grunze</name></author><author><name sortKey="Gessler, Arthur" sort="Gessler, Arthur" uniqKey="Gessler A" first="Arthur" last="Gessler">Arthur Gessler</name></author><author><name sortKey="Weiss, Michael" sort="Weiss, Michael" uniqKey="Weiss M" first="Michael" last="Weiss">Michael Weiss</name></author><author><name sortKey="Nehls, Uwe" sort="Nehls, Uwe" uniqKey="Nehls U" first="Uwe" last="Nehls">Uwe Nehls</name></author></analytic><series><title level="j">The New phytologist</title><idno type="ISSN">0028-646X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Biological Transport (MeSH)</term><term>Cation Transport Proteins (genetics)</term><term>Crosses, Genetic (MeSH)</term><term>DNA Primers (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Plant Proteins (genetics)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Populus (growth & development)</term><term>Populus (microbiology)</term><term>Quaternary Ammonium Compounds (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Composés d'ammonium quaternaire (métabolisme)</term><term>Croisements génétiques (MeSH)</term><term>Mycorhizes (physiologie)</term><term>Populus (croissance et développement)</term><term>Populus (microbiologie)</term><term>Protéines végétales (génétique)</term><term>RT-PCR (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Symbiose (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Transport biologique (MeSH)</term><term>Transporteurs de cations (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cation Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term><term>Transporteurs de cations</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Quaternary Ammonium Compounds</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Composés d'ammonium quaternaire</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycorhizes</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Biological Transport</term><term>Crosses, Genetic</term><term>DNA Primers</term><term>Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Croisements génétiques</term><term>RT-PCR</term><term>Réaction de polymérisation en chaîne</term><term>Symbiose</term><term>Séquence nucléotidique</term><term>Transport biologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">One way to elucidate whether ammonium could act as a nitrogen (N) source delivered by the fungus in ectomycorrhizal symbiosis is to investigate plant ammonium importers. Expression analysis of a high-affinity ammonium importer from Populus tremulax tremuloides (PttAMT1.2) and of known members of the AMT1 gene family from Populus trichocarpa was performed. In addition, PttAMT1.2 function was studied in detail by heterologous expression in yeast. PttAMT1.2 expression proved to be root-specific, affected by N nutrition, and strongly increased in a N-independent manner upon ectomycorrhiza formation. The corresponding protein had a K(M) value for ammonium of c. 52 microm. From the seven members of the AMT1 gene family, one gene was exclusively expressed in roots while four genes were detectable in all poplar organs but with varying degrees of expression. Ectomycorrhiza formation resulted in a strong upregulation of three of these genes. Our results indicate an increased ammonium uptake capacity of mycorrhized poplar roots and suggest, together with the expression of putative ammonium exporter genes in the ectomycorrhizal fungus Amanita muscaria, that ammonium could be a major N source delivered from the fungus towards the plant in symbiosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16313651</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0028-646X</ISSN><JournalIssue CitedMedium="Print"><Volume>168</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Dec</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The high-affinity poplar ammonium importer PttAMT1.2 and its role in ectomycorrhizal symbiosis.</ArticleTitle><Pagination><MedlinePgn>697-706</MedlinePgn></Pagination><Abstract><AbstractText>One way to elucidate whether ammonium could act as a nitrogen (N) source delivered by the fungus in ectomycorrhizal symbiosis is to investigate plant ammonium importers. Expression analysis of a high-affinity ammonium importer from Populus tremulax tremuloides (PttAMT1.2) and of known members of the AMT1 gene family from Populus trichocarpa was performed. In addition, PttAMT1.2 function was studied in detail by heterologous expression in yeast. PttAMT1.2 expression proved to be root-specific, affected by N nutrition, and strongly increased in a N-independent manner upon ectomycorrhiza formation. The corresponding protein had a K(M) value for ammonium of c. 52 microm. From the seven members of the AMT1 gene family, one gene was exclusively expressed in roots while four genes were detectable in all poplar organs but with varying degrees of expression. Ectomycorrhiza formation resulted in a strong upregulation of three of these genes. Our results indicate an increased ammonium uptake capacity of mycorrhized poplar roots and suggest, together with the expression of putative ammonium exporter genes in the ectomycorrhizal fungus Amanita muscaria, that ammonium could be a major N source delivered from the fungus towards the plant in symbiosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Selle</LastName><ForeName>Anita</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Eberhard-Karls-Universität, Physiologische Okologie der Pflanzen, Auf der Morgenstelle 1, D-72076 Tübingen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Willmann</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Grunze</LastName><ForeName>Nina</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Gessler</LastName><ForeName>Arthur</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Weiss</LastName><ForeName>Michael</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Nehls</LastName><ForeName>Uwe</ForeName><Initials>U</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D027682">Cation Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant 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MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>11</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>4</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>11</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16313651</ArticleId><ArticleId IdType="pii">NPH1535</ArticleId><ArticleId IdType="doi">10.1111/j.1469-8137.2005.01535.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Tübingen</li></region><settlement><li>Tübingen</li></settlement></list><tree><noCountry><name sortKey="Gessler, Arthur" sort="Gessler, Arthur" uniqKey="Gessler A" first="Arthur" last="Gessler">Arthur Gessler</name><name sortKey="Grunze, Nina" sort="Grunze, Nina" uniqKey="Grunze N" first="Nina" last="Grunze">Nina Grunze</name><name sortKey="Nehls, Uwe" sort="Nehls, Uwe" uniqKey="Nehls U" first="Uwe" last="Nehls">Uwe Nehls</name><name sortKey="Weiss, Michael" sort="Weiss, Michael" uniqKey="Weiss M" first="Michael" last="Weiss">Michael Weiss</name><name sortKey="Willmann, Martin" sort="Willmann, Martin" uniqKey="Willmann M" first="Martin" last="Willmann">Martin Willmann</name></noCountry><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Selle, Anita" sort="Selle, Anita" uniqKey="Selle A" first="Anita" last="Selle">Anita Selle</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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